Conversion of glucose to glucose 6 phosphate:

To Do: Create the model in Stella*. Use the mass action kinetics to describe the rate of reaction.
Run simulations with each of the parameter values indicated. Monitor the amount of glucose produced over time for each simulation experimental condition.

Kinetics: Mass action or Mass flux; Michaelis Menten

Enzyme: hexokinase (glucokinase)

System statements (disclaimer): ATP not taken into account.

 

Reaction Kinetics (Rate law) Kinetic type Constants
Michaelis-Menten

V

Km
k*Glucose irreversible mass action k

 

Parameter Value (refs.) Variable Concentrations mM (refs.)
Vmax (k) 0.84
51.7
68.5
242.8

(1)
(3)
(15)
(17)

Glucose 0.134
0.345
109 		(15)
(14)
(13)
Km 0.08 (1) Glucose 6-phosphate

1.011
1.51

(15)
(1)

Worksheet for classroom work.

Use the "Interface" window to attach a slider to the rate constant for hexokinase (k1) and an input knob for the concentration of glucose. Set the range of values for the slider and input knob to encompass the experimentally identified values given below in the parameter table. The input control knobs in Stella allow you to change the initial conditions of the simulation without having to open each object within the model.