Introduction to Stella: Done as Live Presentation

Directions

Work in groups of 3. Work with people from different discipline. The goal of the lab is to become familiar with the software and the process of constructing a model.

To be performed

A. Draw flow diagrams/concept map for the statements provided below.

1.Create a concept map in stella that model of the following:

* inactive MPF becomes active MPF
* Active MPF becomes inactive MPF

2.Concept map the following:

* Cyclin is synthesized and degraded
* Cyclin stimulates inactive MPF to become active MPF

B. Create a concept map in Stella.

C. This is designed for you to try multiple scenarios of reaction rates and variable “concentrations”. The variable values are arbitrary and assumed to have the same units. There is no correct value in this exercise. Do you get different behaviors? Assume the following values for reactions and variables and enter them to your Stella model:

1. All reactions are linear and based on the law of mass action (rate constant x substrate).

2. Values for cyclin and MPF range from 0.1 to 2. Choose at least three values to examine.

3. Use the at least two of following set of rate constants: 0.05, 0.1, 1.0, 2.0

Write notes containing the following:

1. Describe the characteristics of at least one set of simulation results.
2. Describe briefly why you think you obtained the results you did?
3. Is there something you need that you did not have for this lab?
4. What questions came up if any?